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In most cases, tissue is cooled to between 80 and 140 and then placed into liquid nitrogen for storage [29, 5557]

Researchers observed that Puenarin treatment alleviated the crypt deformation, inflammatory cell infiltration and reduced the pro-inflammatory cytokine markers in the colon mucosa of mice and well act as a potent therapeutic agent that could be used in UC for maintaining gut microbiota homeostasis [131]